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Core responsible: Prof
Joakim Lundeberg (joalun@kth.se)
Contact Person: Research Engineer Frida Lindström
(frida.lindstrom@biotech.kth.se)
| Objectives:
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• |
Sequence Capture - Target enrichment before
sequencing. |
| • |
Gene Expression Service on Agilent
microarrays. |
| Description: |
| KTH Microarray Center is part of KTH Genome Center, which
is a SWEDAC accredited unit. |
Sequence Capture:
NimbleGen Sequence Capture arrays enable you to produce
targeted, sequencing-ready samples for use with next-generation
sequencing instruments like the Genome Sequencer FLX System
from 454 Life Sciences. Utilizing high-density, long-oligo
NimbleGen arrays, either the whole human exome or the human
genomic regions you specify are hybridized and eluted, ready
for subsequent amplification and sequencing. For the sequencing
part see Sequencing Center.
It is important to keep in mind that the customer designs
and buy their own arrays from NimbleGen and send them to
us. The cost for the NimbleGen Sequence Capture is depending
on the number of samples, protocol etc. We can provide you
a quote after initial discussions. |
| Gene Expression Service:
The research community can submit RNA samples to be analyzed
on Agilent commercially produced microarrays. The RNA
is quality controlled followed by amplification, labeling
and hybridization. The hybridized arrays are scanned and
image analysis is performed. It is important to keep in
mind that we do not provide the analysis of the data.
For users who need help with thorough data analysis we
recommend ArrayAdvice, (www.arrayadvice.se
or info@arrayadvice.se).
The cost for the Gene Expression Service is depending
on the number of samples, your choice of array and protocol.
We can provide you a quote after initial discussions.
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